Sokolov V S, Chernyĭ V V, Markin V S
Biofizika. 1984 May-Jun;29(3):424-9.
The inner field compensation method was used for the measurement of the boundary dipole potentials as a function of phloretin and fluorescein concentration, ionic strength and pH. These potentials were compared with calculated from the conductance change in the presence of nonactin. Both methods gave similar results for fluorescein, but the steady-state potentials in the case of phloretin, obtained by the first method were smaller. These results imply that fluorescein, being introduced on one side of the BLM, remained on that side contrary to phloretin which penetrated through the membrane and partitioned between its both boundaries.
采用内场补偿法测量边界偶极子电位,该电位是根皮素、荧光素浓度、离子强度和pH的函数。将这些电位与在缬氨霉素存在下由电导变化计算得到的结果进行比较。两种方法对荧光素给出了相似的结果,但第一种方法得到的根皮素稳态电位较小。这些结果表明,荧光素被引入BLM的一侧后,仍留在该侧,而根皮素则穿透膜并分布在其两侧边界之间。