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通过测量解链曲线下的面积来测定DNA片段的核苷酸含量

[Determination of the nucleotide content of DNA blocks by measuring the area under the melting curve].

作者信息

Vardapetian R R, Vardevanian P O, Panosian G A

出版信息

Biofizika. 1984 May-Jun;29(3):489-91.

PMID:6466726
Abstract

A method for determining the nucleotide content of DNA blocks from areas under the melting curve is suggested. It is shown that this method is more simple and the total melting curve is decomposed not to the Gauss components, but it is considered as as superposition of the melting curves of individual blocks. Application of the method for the determination of calf thymus DNA is discussed.

摘要

提出了一种根据解链曲线下的面积来确定DNA片段核苷酸含量的方法。结果表明,该方法更为简单,并且总解链曲线不是分解为高斯分量,而是被视为各个片段解链曲线的叠加。讨论了该方法在测定小牛胸腺DNA中的应用。

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