Ledenev A N, Tverdokhlebov E N, Davydov R M
Biofizika. 1984 May-Jun;29(3):491-2.
Kinetics of cytochrome P-450 reduction with methylviologen radicals was investigated by flash-photolysis. Formation of carboxycomplex of reduced protein and effect of temperature and substrates of the I type on this reaction were studied. It has been shown that CO addition to protein proceeds in two stages. With temperature increase (20-45 degrees C) and when benzphetamine or hexabarbital are added to solution the weight of the first (faster) stage increases. Such regularity is easily explained by the presence in solution of two conformers of oxidized cytochrome P-450 (with different reactivity) whose equilibrium depends on temperature and presence of the substrate.
通过闪光光解研究了细胞色素P - 450与甲基紫精自由基还原的动力学。研究了还原蛋白羧基配合物的形成以及温度和I型底物对该反应的影响。结果表明，向蛋白中添加一氧化碳分两个阶段进行。随着温度升高（20 - 45摄氏度）以及当向溶液中添加苄非他明或己巴比妥时，第一阶段（较快）的比重增加。这种规律性很容易用溶液中氧化型细胞色素P - 450的两种构象（具有不同反应活性）的存在来解释，其平衡取决于温度和底物的存在。
