Melkonian V Z, Shaldzhian A A, Nalbandian R M
Biokhimiia. 1984 Jun;49(6):928-32.
A novel procedure for purification of copper-thionein from rabbit liver was developed. The molecular mass, copper, and thiol contents were determined. The properties of the protein were compared with those of Cd-thionein. Copper was removed from Cu-thionein and the reconstitution was achieved. The binding of copper leads to an increase in absorbance at 260 nm.
开发了一种从兔肝中纯化铜硫蛋白的新方法。测定了其分子量、铜含量和巯基含量。将该蛋白质的性质与镉硫蛋白的性质进行了比较。从铜硫蛋白中去除铜并实现了重组。铜的结合导致260nm处吸光度增加。
