Smirnov A A
Biull Eksp Biol Med. 1984 Aug;98(8):249-52.
A method for preparing liposomes containing Na3Ca-14C-DTPA by means of phase-reverse evaporation and freezing-thawing is described. Freezing-thawing is required for transformation of the system formed after phase-reverse evaporation at a lipid concentration of over 20-30 mg/ml to liposomal suspension. Liposomes thus obtained contain 10-14 microliters aqueous phase per mumol phosphatidylcholine, their diameter varies between 0.2 and 1.5 micron (the mean value 0.45-0.54 micron). Increasing the concentration of lipids, it is possible to include into liposomes up to 70-80% of 14C-DTPA in solution. The method is believed to be fit for preparing the liposomal forms of other drug hydrophilic compounds.
描述了一种通过相逆蒸发和冻融法制备含Na3Ca-14C-DTPA脂质体的方法。当脂质浓度超过20-30mg/ml时，相逆蒸发后形成的体系需经冻融转化为脂质体悬浮液。如此获得的脂质体每微摩尔磷脂酰胆碱含有10-14微升水相，其直径在0.2至1.5微米之间变化（平均值为0.45-0.54微米）。增加脂质浓度，可使溶液中高达70-80%的14C-DTPA包封于脂质体中。该方法被认为适用于制备其他药物亲水性化合物的脂质体形式。
