Grau-Wagemans M P, Selak I, Lefebvre P P, Moonen G
Brain Res. 1984 Jul;317(1):11-9. doi: 10.1016/0165-3806(84)90135-4.
Cerebellar macroneurones survive and differentiate for at least 10 days in Eagle's minimum essential medium with insulin as the only supplement when cultured either as microexplants or in high-density dissociated cultures, while they do not survive if cultured in low density. The survival is related to the extracellular release of neuronotrophic factor(s). Using a quantitative bioassay of the neuronotrophic effect, it is possible to demonstrate that the effect is concentration dependent. The analysis of the dose-response curves suggests that the neuronotrophic activity is associated with a neuronotoxic activity. The two activities can be segregated using a simple physical method, allowing direct demonstration of the neuronotoxic activity.
当作为微组织块培养或以高密度解离培养时，小脑大神经元在仅添加胰岛素作为补充物的伊格尔氏最低限度基本培养基中存活并分化至少10天，而在低密度培养时则不能存活。其存活与神经营养因子的细胞外释放有关。使用神经营养效应的定量生物测定法，可以证明该效应是浓度依赖性的。剂量反应曲线分析表明，神经营养活性与神经毒性活性相关。这两种活性可以通过一种简单的物理方法分离，从而直接证明神经毒性活性。
