Leblanc P, Gerber G E
Can J Biochem Cell Biol. 1984 Jun;62(6):375-8. doi: 10.1139/o84-052.
The photoreactive omega-diazirinophenoxy derivatives of nonanoate, undecanoate, tridecanoate, and pentadecanoate were shown to be activated by rat liver microsomes to the corresponding acyl-CoA derivatives. The Km and Vmax for these fatty acid analogues were determined: the values obtained indicate that the addition of a photoreactive group to an alkyl chain has an effect similar to that of elongation of the chain by about seven carbons. Incubation of microsomes in the presence of lysophospholipids resulted in the incorporation of the photoreactive fatty acids into the corresponding phospholipids. The ability of mammalian systems to utilize these photoreactive fatty acids for phospholipid synthesis establishes their suitability as photoaffinity analogues of fatty acids.
壬酸酯、十一酸酯、十三酸酯和十五酸酯的光反应性ω-重氮苯氧基衍生物经大鼠肝微粒体激活后可生成相应的酰基辅酶A衍生物。测定了这些脂肪酸类似物的米氏常数（Km）和最大反应速度（Vmax）：所得数值表明，在烷基链上添加一个光反应性基团所产生的效果类似于将链延长约七个碳原子。在溶血磷脂存在的情况下孵育微粒体，会导致光反应性脂肪酸掺入相应的磷脂中。哺乳动物系统利用这些光反应性脂肪酸进行磷脂合成的能力表明它们适合作为脂肪酸的光亲和类似物。
