Thomas P, Wofford H W
Comp Biochem Physiol B. 1984;78(2):473-9. doi: 10.1016/0305-0491(84)90061-0.
Plasma corticosteroids of eighteen vertebrate species were separated by reversed-phase high-performance liquid chromatography (HPLC). The major corticosteroids identified in these species by HPLC agreed with previous reports which used vigorous criteria for identification. The HPLC technique was validated for the assay of cortisol in mullet plasma and corticosterone in the rat. Qualitative as well as quantitative changes in corticosteroid secretion were detected by HPLC in several vertebrates during exposure to stress.
采用反相高效液相色谱法（HPLC）对18种脊椎动物的血浆皮质类固醇进行了分离。通过HPLC在这些物种中鉴定出的主要皮质类固醇与先前使用严格鉴定标准的报告一致。HPLC技术已针对鲻鱼血浆中的皮质醇和大鼠中的皮质酮测定进行了验证。在几种脊椎动物暴露于应激期间，通过HPLC检测到了皮质类固醇分泌的定性和定量变化。
