从鸡胚脑、肌肉和皮肤中分离α和β微管蛋白。
Isolation and separation of alpha and beta-tubulin from chick-embryo brain, muscle and skin.
作者信息
el-Hamalawi A R
出版信息
Biochem J. 1978 Mar 1;169(3):717-9. doi: 10.1042/bj1690717.
Abstract
Two kinds of tubulin, alpha and beta, have been described in microtubules from many different systems. In the present study a new method is described for isolating and separating these two kinds of tubulin from chick-embryo brain, muscle and skin. The isolated tubulins were characterized by polyacrylamide-gel disc electrophoresis in the presence of urea and sodium dodecyl sulphate.
摘要
在许多不同系统的微管中已发现两种微管蛋白，即α微管蛋白和β微管蛋白。在本研究中，描述了一种从鸡胚脑、肌肉和皮肤中分离并区分这两种微管蛋白的新方法。通过在尿素和十二烷基硫酸钠存在的情况下进行聚丙烯酰胺凝胶圆盘电泳对分离出的微管蛋白进行表征。
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Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.微管蛋白α亚基的异质性以及单个生物体中微管蛋白的变异性。
J Cell Biol. 1976 May;69(2):301-12. doi: 10.1083/jcb.69.2.301.
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