小鼠肝脏三种胞质核糖核酸酶的纯化及性质

Purification and properties of three cytosolic ribonucleases of mouse liver.

作者信息

Mavrothalassitis G J, Georgatsos J G

出版信息

Eur J Biochem. 1984 Aug 1;142(3):481-6. doi: 10.1111/j.1432-1033.1984.tb08311.x.

DOI:10.1111/j.1432-1033.1984.tb08311.x

Abstract

The ribonucleolytic activity of mouse liver cytosol is due to at least three different enzymes, whose purification is reported. Two of these enzymes, an alkaline and a neutral RNase, have specificities practically identical with that of pancreatic RNase. The third enzyme, an acid RNase, is highly specific for NpU bonds where N is A, G or C and also cleaves ApG bonds provided they are part of a GpApG sequence and preferentially a GpApGpA repeat.

摘要

小鼠肝脏胞质溶胶的核糖核酸酶活性至少归因于三种不同的酶，本文报道了它们的纯化过程。其中两种酶，一种碱性核糖核酸酶和一种中性核糖核酸酶，其特异性与胰腺核糖核酸酶几乎相同。第三种酶，一种酸性核糖核酸酶，对N为A、G或C的NpU键具有高度特异性，并且还能切割ApG键，前提是它们是GpApG序列的一部分，并且优先切割GpApGpA重复序列。