Paul D, Piasecki A
Exp Cell Res. 1984 Sep;154(1):95-100. doi: 10.1016/0014-4827(84)90670-0.
Adult rat hepatocytes in primary cultures are stimulated to synthesize DNA in response to rat serum, whereas rat plasma is considerably less active. Biological activity is present in rat platelets and is secreted during aggregation in response to thrombin. The material secreted by rat platelets is heat labile and is sensitive to digestion with trypsin, suggesting that it is a protein. When assayed on 3T3 cells this material also stimulates DNA synthesis; however, the trypsin-sensitive activity is heat stable (100 degrees C, 10 min). These results indicate that rat platelets contain hepatotrophic activities which by virtue of their heat sensitivity are distinct from heat-stable platelet-derived growth factor (PDGF)-like mitogenic activities required by 3T3 cells for growth. It is possible that hepatotrophic platelet factors might be involved in mediating liver regeneration in the rat after partial hepatectomy.
原代培养的成年大鼠肝细胞在受到大鼠血清刺激时会被诱导合成DNA，而大鼠血浆的活性则要低得多。大鼠血小板中存在生物活性，并且在凝血酶刺激聚集过程中会分泌这种活性物质。大鼠血小板分泌的物质对热不稳定，并且对胰蛋白酶消化敏感，这表明它是一种蛋白质。当在3T3细胞上进行检测时，这种物质也能刺激DNA合成；然而，对胰蛋白酶敏感的活性对热稳定（100摄氏度，10分钟）。这些结果表明，大鼠血小板含有肝细胞营养活性，由于其对热敏感，与3T3细胞生长所需的对热稳定的血小板衍生生长因子（PDGF）样促有丝分裂活性不同。肝切除术后大鼠肝脏再生过程中，肝细胞营养性血小板因子可能参与其中起着介导作用。
