钙调蛋白可激活兔心脏质膜中的腺苷酸环化酶。

Calmodulin activates adenylate cyclase from rabbit heart plasma membranes.

作者信息

Panchenko M P, Tkachuk V A

出版信息

FEBS Lett. 1984 Aug 20;174(1):50-4. doi: 10.1016/0014-5793(84)81076-5.

Abstract

It was shown that calmodulin (CM) activates the adenylate cyclase (AC) of rabbit heart light sarcolemma in the presence of micromolar free Ca2+ concentrations and this effect is blocked by trifluoroperazine and troponin I. GTP (in the presence of isoproterenol) and Gpp(NH)p are able to increase the CM-dependent activity of enzyme. It was concluded that there is no special CM-dependent "form' of AC in the heart and the common catalytic component of AC can be regulated both by CM and guanine nucleotide-binding regulatory component (N-protein). In the presence of Ca2+ and guanine nucleotide heart AC exists as a complex: CM-catalytic component-N-protein.

摘要

研究表明，在微摩尔级游离钙离子浓度存在的情况下，钙调蛋白（CM）可激活兔心脏轻肌膜的腺苷酸环化酶（AC），且这种效应可被三氟拉嗪和肌钙蛋白I阻断。GTP（在异丙肾上腺素存在的情况下）和Gpp(NH)p能够增强酶的CM依赖性活性。得出的结论是，心脏中不存在特殊的CM依赖性AC“形式”，AC的常见催化成分可同时受CM和鸟嘌呤核苷酸结合调节成分（N蛋白）的调节。在钙离子和鸟嘌呤核苷酸存在的情况下，心脏AC以CM - 催化成分 - N蛋白复合物的形式存在。