Schlüter K J, André J, Enzmann F, Kerp L
Horm Metab Res. 1984 Aug;16(8):411-4. doi: 10.1055/s-2007-1014805.
The insulin receptor binding of human and pork insulin was measured in four different pork brain tissues. In medial hypothalamus, infundibulum of pituitary gland, neurohypophysis, and adenohypophysis insulin receptors were found. Binding of mono-125I-(TyrA14)-insulin proceeded rapidly at 37 degrees C, pH 7.4, and reached a steady state for human and pork insulin at 15 minutes. Biosynthetic human insulin and semisynthetic human insulin dissociated more rapidly than pork insulin. In the four tested brain tissues the specific insulin binding per mg protein differed significantly (2p less than 0.01). The receptor affinity constant of human insulin was significantly (2p less than 0.01) lower in comparison to pork insulin in the four brain tissues studied. This study demonstrates that there are insulin receptors in pork brain tissue, that the amounts of these receptors vary depending on the area and that the brain insulin receptors react differently to pork and human insulin.
在四种不同的猪脑组织中测量了人胰岛素和猪胰岛素的受体结合情况。在内侧下丘脑、垂体漏斗、神经垂体和腺垂体中发现了胰岛素受体。单 -125I-(酪氨酸A14)-胰岛素在37℃、pH 7.4条件下结合迅速，15分钟时人胰岛素和猪胰岛素均达到稳态。生物合成人胰岛素和半合成人胰岛素比猪胰岛素解离更快。在四个测试的脑组织中，每毫克蛋白质的特异性胰岛素结合量差异显著（P<0.01）。在所研究的四个脑组织中，人胰岛素的受体亲和常数与猪胰岛素相比显著较低（P<0.01）。本研究表明，猪脑组织中存在胰岛素受体，这些受体的数量因区域而异，并且脑胰岛素受体对猪胰岛素和人胰岛素的反应不同。
