Takasaki S, Murray G J, Furbish F S, Brady R O, Barranger J A, Kobata A
J Biol Chem. 1984 Aug 25;259(16):10112-7.
The N-asparagine-linked oligosaccharide chains of homogeneous human placental beta-glucocerebrosidase were released by hydrazinolysis, and their structures were analyzed. The sequence of sugars, linkage, and anomeric configuration of the glycosidic bonds were determined. Approximately 20% of the released sugar chain was of the typical high mannose type. The balance was of complex type with biantennary and triantennary structures. As a major component, the following trisialylated oligosaccharide was detected: (formula; see text).
通过肼解作用释放了纯化的人胎盘β-葡萄糖脑苷脂酶的N-天冬酰胺连接的寡糖链，并对其结构进行了分析。确定了糖的序列、连接方式以及糖苷键的异头构型。释放的糖链中约20%为典型的高甘露糖型。其余为具有双天线和三天线结构的复合型。作为主要成分，检测到以下三唾液酸化寡糖：（分子式；见正文）。
