用于分析糖胺聚糖还原端己糖醛酸的改进方法。

Improved analytical method for the hexuronic acids at the reducing terminals of glycosaminoglycans.

作者信息

Majima M, Nakamura T, Igarashi S, Matsue H, Endo M

出版信息

J Biochem Biophys Methods. 1984 Jul;9(3):245-9. doi: 10.1016/0165-022x(84)90029-0.

Abstract

When endo-uronidases act on glycosaminoglycans, the reaction products have hexuronic acid residues at the reducing terminals. An analytical method for hexuronic acids at the reducing terminals was devised for hyaluronate oligosaccharides having hexuronic acid residues at the reducing terminals. The procedure is as follows: Hexuronic acid residues at the reducing terminals of hyaluronate oligosaccharides were tritiated with reduction using NaB[3H]4 and the products were hydrolyzed with trifluoroacetic acid and nitrous acid. As a result, the tritiated and reduced hexuronic acid residues, that is aldonic acids, were liberated from the reducing terminals. After passing them through anion and cation ion-exchange resins, the aldonic acids were lactonized. The lactones were developed on paper chromatography, and their radioactivities determined on the paper. The method is also useful for discrimination between glucuronic acid and iduronic acid at the reducing terminals of glycosaminoglycans.

摘要

当内切糖醛酸酶作用于糖胺聚糖时，反应产物在还原末端具有己糖醛酸残基。针对在还原末端具有己糖醛酸残基的透明质酸寡糖，设计了一种用于分析还原末端己糖醛酸的方法。步骤如下：使用NaB[3H]4通过还原反应将透明质酸寡糖还原末端的己糖醛酸残基进行氚化标记，然后产物用三氟乙酸和亚硝酸水解。结果，氚化标记且还原的己糖醛酸残基，即醛糖酸，从还原末端释放出来。使其通过阴离子和阳离子交换树脂后，醛糖酸被内酯化。内酯在纸色谱上展开，并测定纸上的放射性。该方法对于区分糖胺聚糖还原末端的葡萄糖醛酸和艾杜糖醛酸也很有用。