Orikasa M, Iwase I, Kozima K, Shimizu F
J Gen Microbiol. 1984 Jul;130(7):1757-63. doi: 10.1099/00221287-130-7-1757.
Antigenic substances from livers of mice infected with Tyzzer's disease were purified by means of sucrose density gradient zonal centrifugation and affinity column chromatography using antiserum and checking antigenicity with the complement fixation test. Fractions obtained from zonal centrifugation fell into three main groups with different molecular weights, two of which (Fr. I and Fr. II) positively reacted with antiserum in the complement fixation tests. Both fractions were further purified by affinity column chromatography. The molecular weights of the main antigenic substances derived from Fr. I and Fr. II were determined to be about 52 000 and 66 000, respectively, by means of SDS-PAGE.
通过蔗糖密度梯度区带离心法和亲和柱色谱法，利用抗血清对感染泰泽氏病的小鼠肝脏中的抗原物质进行纯化，并通过补体结合试验检测抗原性。区带离心得到的组分分为分子量不同的三个主要组，其中两组（组分I和组分II）在补体结合试验中与抗血清呈阳性反应。两组分均通过亲和柱色谱法进一步纯化。通过SDS-PAGE测定，源自组分I和组分II的主要抗原物质的分子量分别约为52000和66000。
