Stadnichuk I N, Abdurakhmanov I A, Kuznetsova N Iu, Erokhin Iu E
Mol Biol (Mosk). 1984 May-Jun;18(3):685-90.
The light-harvesting bacteriochlorophyll-protein complex B850 has been isolated from two species of purple bacteria, Rhodopseudomonas palustris and Chromatium minutissimum. Absorption and fluorescence spectra at 20 degrees and--196 degrees C of this complex were registered. Second derivatives of the absorption spectra, Stepanov's relation and computer curve analyses in terms of asymmetrical Gaussian components show that absorption spectra consist of five and fluorescence spectra--of three components. These components were analysed in terms of exciton interaction among bacteriochlorophyll molecules. Data obtained were used for building-up of the molecular model of the complex.
捕光细菌叶绿素蛋白复合物B850已从两种紫色细菌——沼泽红假单胞菌和极小色杆菌中分离出来。记录了该复合物在20℃和-196℃下的吸收光谱和荧光光谱。吸收光谱的二阶导数、斯捷潘诺夫关系以及基于不对称高斯分量的计算机曲线分析表明，吸收光谱由五个分量组成，荧光光谱由三个分量组成。根据细菌叶绿素分子间的激子相互作用对这些分量进行了分析。所获得的数据用于构建该复合物的分子模型。
