Nikolaev L G, Glotov B O, Dashkevich V K, Barbashov S F, Severin E S
Mol Biol (Mosk). 1984 May-Jun;18(3):736-42.
Mutual arrangement of histone H1 molecules was studied in calf thymus nuclei, extended chromatin and chromatin, isolated and kept in 8 M urea. Histone H1 dimers crosslinked with methyl 4-mercaptobutyrimidate were digested with chymotrypsin and crosslinked fragments obtained were analysed by diagonal gel electrophoresis. In all chromatins tested the N- and C-terminal parts of the H1 molecules were crosslinked in all possible combinations, i.e. C-C, C-N and N-N. These and related data obtained earlier indicate, that the proximity of histone H1 molecules in chromatin is determined by the structure of nucleosomal chain itself and not by chromatin superstructure. The results also suggest that the H1A and H1B subfractions of histone H1 are interspersed in extended nucleosomal chains.
研究了小牛胸腺细胞核、伸展染色质以及分离并保存在8M尿素中的染色质中组蛋白H1分子的相互排列。用糜蛋白酶消化与4-巯基丁酸甲酯交联的组蛋白H1二聚体，并通过对角线凝胶电泳分析得到的交联片段。在所有测试的染色质中，H1分子的N端和C端部分以所有可能的组合交联，即C-C、C-N和N-N。这些以及早期获得的相关数据表明，染色质中组蛋白H1分子的接近程度由核小体链本身的结构决定，而非由染色质超结构决定。结果还表明，组蛋白H1的H1A和H1B亚组分在伸展的核小体链中是穿插分布的。
