Kir'ianov G I, Smirnova T A, Manamsh'ian T A, Khodosovskaia A M
Biokhimiia. 1987 Dec;52(12):1983-9.
The content of histone H1 (H1/H4 ratio) in dinucleosomes with the DNA of various length liberated from L-cell nuclear chromatin by micrococcal nuclease was analyzed. It was found that the histone H1 content in the dichromatosome is two times as low as that in the largest dinucleosome and in the complete mononucleosome. The set of chromatin fragments liberated from the Triton X-100 pretreated nuclei differs considerably from that of chromatin sites devoid of histone H1 (the de novo replicating chromatin and the chromatin formed on the undermethylated DNA). A scheme for asymmetric distribution of histone H1 with molecules oriented along the nucleosomal fibril, which reflects the peculiarities of chromatin fragmentation by micrococcal nuclease with predominant liberation of the dichromatosome, is proposed.
分析了通过微球菌核酸酶从L细胞核染色质中释放出的具有不同长度DNA的双核小体中组蛋白H1的含量（H1/H4比率）。发现双核心小体中组蛋白H1的含量比最大的双核小体和完整单核小体中的低两倍。从经Triton X - 100预处理的细胞核中释放出的染色质片段组与不含组蛋白H1的染色质位点（从头复制染色质和在低甲基化DNA上形成的染色质）有很大差异。提出了一种组蛋白H1沿核小体纤维定向分布的不对称方案，该方案反映了微球菌核酸酶切割染色质的特点，其中双核心小体的释放占主导。
