Bronk B V, Humphries E H, Rupert C S
Mutat Res. 1984 Jul-Aug;132(1-2):33-9. doi: 10.1016/0167-8817(84)90064-6.
A survival assay with chick-embryo fibroblasts was used to study photoreactivation of ultraviolet (UV) irradiation-induced damage. The kinetics of the photoreactivation was studied as a function of the length of a post UV dark incubation period of from 0 to 18 h at 38.5 degrees C. The logarithmic survival curve with no photoreactivation had a Do of about 4.3 J/m2 giving approximately 0.8% relative plating efficiency after a UV dose of 21 J/m2. At this dose the efficiency of photoreactivation (survival increase per unit blacklight dose) increased with post UV incubation time reaching a maximum at 4-6 h, then declining until there was little photoreactivation observed for times longer than about 11 h. The possibility that this effect was produced by pre-UV perturbations of the cell cycle was eliminated by the fact that the same results were achieved after several rather different trypsinization protocols. The shape of the photoreactivation vs. blacklight curve at the time of peak efficiency showed a threshold up to about 3 kJ/m2, a rising portion and a plateau after 12-16 kJ/m2 when the survival increased by a factor of roughly 8.
利用鸡胚成纤维细胞存活试验研究紫外线（UV）照射所致损伤的光复活作用。研究了光复活动力学与在38.5℃下UV照射后黑暗孵育时间（0至18小时）的函数关系。无光复活作用时的对数存活曲线的Do约为4.3 J/m²，在21 J/m²的UV剂量后相对铺板效率约为0.8%。在此剂量下，光复活效率（每单位黑光剂量的存活增加）随UV照射后的孵育时间增加，在4至6小时达到最大值，然后下降，直到孵育时间超过约11小时时几乎观察不到光复活作用。细胞周期的UV照射前扰动产生这种效应的可能性被排除，因为在几种相当不同的胰蛋白酶消化方案后都得到了相同的结果。在效率峰值时，光复活与黑光曲线的形状显示出高达约3 kJ/m²的阈值、上升部分以及在12至16 kJ/m²后出现的平台期，此时存活增加约8倍。
