Goldstein L S
Mutat Res. 1984 Aug;140(4):193-7. doi: 10.1016/0165-7992(84)90076-9.
Male mice were treated with nitrogen mustard (2.25 mg/kg), procarbazine (100 mg/kg) or vincristine (0.67 mg/kg). 5 weeks later they were mated to untreated females and the dominant lethal mutant rate was determined in the offspring by an in vitro analysis. Nitrogen mustard and procarbazine induced dominant lethal mutations to significant levels. Mutations were expressed, throughout early development, especially as a failure to form a trophectoderm outgrowth and to differentiate and proliferate an inner cell mass. Variations in the dominant lethal mutant rate were found from one week to the next, suggesting that differentiated and stem cell spermatogonia have differential sensitivity to mutation induction and cell killing by each drug.
雄性小鼠接受了氮芥（2.25毫克/千克）、甲基苄肼（100毫克/千克）或长春新碱（0.67毫克/千克）的处理。5周后，它们与未处理的雌性小鼠交配，并通过体外分析确定后代中的显性致死突变率。氮芥和甲基苄肼诱导出显著水平的显性致死突变。在整个早期发育过程中都出现了突变，特别是表现为无法形成滋养外胚层生长以及无法分化和增殖内细胞团。在不同周之间发现显性致死突变率存在差异，这表明分化型和干细胞精原细胞对每种药物的突变诱导和细胞杀伤具有不同的敏感性。
