用美索比妥溶解的大鼠脑和心脏线粒体己糖激酶的比较。

Comparison of rat brain and heart mitochondrial hexokinase solubilized by methohexital.

作者信息

Karcher L, Rachman A, Krieglstein J

出版信息

Naunyn Schmiedebergs Arch Pharmacol. 1984 May;326(1):80-2. doi: 10.1007/BF00518783.

Abstract

The purpose of the present investigation was to compare the solubilizing effect of methohexital on the mitochondrially bound hexokinase activity in brain and heart tissue of the rat. Experiments were performed using intact rats, the isolated perfused rat brain and heart as well as mitochondrial fractions from rat brain and heart tissue. It was shown that bound hexokinase activity was significantly solubilized by methohexital in brain tissue in vivo and in the isolated perfused rat brain but no effect was demonstrable in heart tissue. When mitochondrial fractions were incubated with methohexital in vitro, hexokinase activity was significantly solubilized from brain mitochondria but only slightly from heart mitochondria although glucose-6-phosphate was able to displace hexokinase also from heart mitochondria. The results suggest that mitochondrially bound hexokinase activity in brain tissue is particularly sensitive against the solubilizing effect of anesthetics. This effect could contribute to the sensitivity of brain function and metabolism against anesthetic drugs.

摘要

本研究的目的是比较美索比妥对大鼠脑和心脏组织中线粒体结合型己糖激酶活性的增溶作用。实验使用了完整大鼠、离体灌注的大鼠脑和心脏以及大鼠脑和心脏组织的线粒体部分。结果表明，在体内脑组织和离体灌注的大鼠脑中，美索比妥可显著增溶结合型己糖激酶活性，但在心脏组织中未显示出作用。当线粒体部分在体外与美索比妥孵育时，己糖激酶活性从脑线粒体中显著增溶，但从心脏线粒体中仅轻微增溶，尽管葡萄糖-6-磷酸也能使己糖激酶从心脏线粒体中解离。结果表明，脑组织中线粒体结合型己糖激酶活性对麻醉剂的增溶作用特别敏感。这种作用可能有助于脑功能和代谢对麻醉药物的敏感性。