Bryan L J, O'Donnell S R
Naunyn Schmiedebergs Arch Pharmacol. 1984 Jun;326(3):268-72. doi: 10.1007/BF00505329.
Tracheal segments from guinea-pigs pretreated with 6-hydroxydopamine were incubated in 5 or 200 mumol X 1(-1) adrenaline at 37 degrees C. Catechol-O-methyltransferase and monoamine oxidase were inhibited by U-0521 and pargyline, respectively, and the accumulation of adrenaline in trachealis smooth muscle cells was measured by fluorescence microphotometry. The effects of anoxia, anoxia plus glucose deprivation, ouabain, reduced sodium (Na+) concentration, elevated potassium (K+) concentration and calcium (Ca2+) deprivation on adrenaline accumulation were examined in tissues incubated in adrenaline for 5 min. Adrenaline accumulation in the trachealis smooth muscle cells was reduced by inhibition of oxidative metabolism (anoxia and glucose deprivation), by ouabain (100 mumol X 1(-1), by a reduction in Na+ concentration from 139 mmol X 1(-1) to 25 mmol X 1(-1), and by increases in K+ concentration, but not by omission of Ca2+. These results from guinea-pig trachealis smooth muscle cells, in combination with those obtained by other workers on various organs and tissues, indicate that the extraneuronal accumulation of catecholamines requires energy derived from oxidative metabolism and is very sensitive to inhibition by increases in K+ concentration. However, there is marked variability in the effects of ouabain and of reduced Na+ or Ca2+ concentrations on the accumulation.
将用6-羟基多巴胺预处理过的豚鼠气管段在37℃下于5或200μmol·L⁻¹肾上腺素中孵育。儿茶酚-O-甲基转移酶和单胺氧化酶分别被U-0521和帕吉林抑制，通过荧光显微光度法测量气管平滑肌细胞中肾上腺素的蓄积。在肾上腺素中孵育5分钟的组织中，检测了缺氧、缺氧加葡萄糖剥夺、哇巴因、降低钠（Na⁺）浓度、升高钾（K⁺）浓度和钙（Ca²⁺）剥夺对肾上腺素蓄积的影响。气管平滑肌细胞中的肾上腺素蓄积因氧化代谢抑制（缺氧和葡萄糖剥夺）、哇巴因（100μmol·L⁻¹）、Na⁺浓度从139mmol·L⁻¹降至25mmol·L⁻¹以及K⁺浓度升高而降低，但不因Ca²⁺缺失而降低。豚鼠气管平滑肌细胞的这些结果，与其他研究人员在各种器官和组织上获得的结果相结合，表明儿茶酚胺的非神经元性蓄积需要氧化代谢产生的能量，并且对K⁺浓度升高的抑制非常敏感。然而，哇巴因以及降低Na⁺或Ca²⁺浓度对蓄积的影响存在明显差异。
