Hery M, Faudon M, Hery F
Peptides. 1984 Mar-Apr;5(2):313-7. doi: 10.1016/0196-9781(84)90226-2.
The effect of vasoactive intestinal peptide (VIP) on spontaneous and induced release of newly synthesized 5-hydroxytryptamine (5-HT) was studied in the suprachiasmatic area (SCA) using a superfusion system. To test the possible modulation by E2 on the interaction VIP-5-HT, the experiments were conducted on male, ovariectomized (OVX) and ovariectomized oestradiol implanted rats (OVX-E2). VIP (10(-7)M) infused for 15 min caused an increase of 5-HT release from SCA of male and OVX. The positive effect of VIP on 5-HT release results partially from an inhibition of the reuptake of 5-HT: in male and OVX SCA, VIP inhibited the 3H-5-HT uptake by 40 to 50%. The infusion of VIP before a pulse of K+ (10-20-30-56 mM) leads to a potentialisation of the evoked release suggesting that VIP sensitized the presynaptic membrane to the process linking depolarization and release. When SCA taken from OVX-E2 were exposed to VIP, 5-HT uptake and consequently 5-HT release were unchanged. The present results suggest that the metabolism of 5-HT in the SCA is influenced by VIP and that this regulation may be modulated by E2. This interaction between E2, VIP and 5-HT at the SCA level may be involved in the regulation of phasic LH and prolactin surge.
采用灌流系统，研究了血管活性肠肽（VIP）对视交叉上核（SCA）中新合成的5-羟色胺（5-HT）自发释放和诱导释放的影响。为了测试E2对VIP-5-HT相互作用的可能调节作用，实验在雄性、去卵巢（OVX）和去卵巢并植入雌二醇的大鼠（OVX-E2）上进行。输注15分钟的VIP（10^-7M）导致雄性和OVX大鼠SCA中5-HT释放增加。VIP对5-HT释放的积极作用部分源于对5-HT再摄取的抑制：在雄性和OVX大鼠的SCA中，VIP抑制3H-5-HT摄取达40%至50%。在给予K+（10 - 20 - 30 - 56 mM）脉冲之前输注VIP会导致诱发释放增强，这表明VIP使突触前膜对将去极化与释放联系起来的过程敏感化。当将取自OVX-E2大鼠的SCA暴露于VIP时，5-HT摄取以及因此的5-HT释放未发生变化。目前的结果表明，SCA中5-HT的代谢受VIP影响，并且这种调节可能受E2调节。E2、VIP和5-HT在SCA水平的这种相互作用可能参与了LH和催乳素阵发性高峰的调节。
