Fomenko L A, Kuznetsova E A, Gaziev A I
Radiat Environ Biophys. 1984;23(3):223-33. doi: 10.1007/BF01213224.
The effect of mercaptoethylamine (MEA) on degradation of DNA in thermophilic bacteria Bac. stear. exposed to gamma-, UV-rays or methylnitrosourea (MNU) was studied. Using centrifugation on alkaline and neutral sucrose gradients, it was shown that MEA inhibits the accumulation of breaks in the DNA of Bac. stear. It also lowers the level of DNA degradation in toluene-treated cells of Bac. stear. under the action of the intrinsic nuclease, reduces the activity of the endonuclease specific for apurinic DNA, as well as that of S1-nuclease and DNase-I in vitro. The inhibition in the accumulation of DNA breaks is assumed to be due to a decrease of the endonuclease activity in the cells of thermophilic bacteria.
研究了巯基乙胺（MEA）对嗜热脂肪芽孢杆菌（Bac. stear.）在受到γ射线、紫外线或甲基亚硝基脲（MNU）照射时DNA降解的影响。通过在碱性和中性蔗糖梯度上进行离心，结果表明MEA抑制嗜热脂肪芽孢杆菌DNA断裂的积累。它还降低了嗜热脂肪芽孢杆菌经甲苯处理的细胞在内在核酸酶作用下的DNA降解水平，在体外降低了对脱嘌呤DNA特异的内切核酸酶以及S1核酸酶和DNase-I的活性。DNA断裂积累的抑制被认为是由于嗜热细菌细胞内内切核酸酶活性的降低。
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