Shinoda H, Ogura H
Calcif Tissue Res. 1978 Feb 28;25(1):75-83. doi: 10.1007/BF02010754.
Sixteen 28-day-old male rats of Wistar strain, with a mean body weight of 179 g, were divided into two equal groups. Each group of eight animals was maintained for 70 days on drinking water, ad lib., containing no fluorine (control group) and 100 ppm of fluorine (experimental group). All specimens examined were obtained from the incisal portions of the incisors. The following types of enamel specimens were prepared for scanning electron microscopy: (1) acid-etched specimens; (2) acid-etched specimens followed by low temperature microincineration; and (3) fractured specimens. The enamel formed during high fluoride exposure showed marked hypocalcification, that is, the crystallite density in the prism core and interprismatic region was lower than that of control animals. The organic substances appeared to increase in these regions. The changes were prominent in the outer and middle enamel layers. Such changes following fluoride administration appear to indicate an inhibition of enamel maturation, that is, an inhibition of the mineral deposition and/or an inhibition of organic matrix withdrawal.
16只28日龄的Wistar品系雄性大鼠，平均体重179克，被分为两组，每组8只。两组动物分别自由饮用不含氟的水（对照组）和含100 ppm氟的水（实验组）70天。所有检测标本均取自门齿的切端部分。制备了以下几种类型的牙釉质标本用于扫描电子显微镜观察：（1）酸蚀标本；（2）酸蚀后进行低温微焚烧的标本；（3）折断标本。高氟暴露期间形成的牙釉质显示出明显的钙化不足，即棱柱核心和柱间质区域的微晶密度低于对照动物。这些区域的有机物质似乎有所增加。这种变化在外层和中层牙釉质层中较为明显。给予氟后出现的这些变化似乎表明牙釉质成熟受到抑制，即矿物质沉积受到抑制和/或有机基质去除受到抑制。
