Manson J M, Murphy M, Richdale N, Smith M K
Toxicology. 1984 Sep 14;32(3):229-42. doi: 10.1016/0300-483x(84)90076-3.
The present study was conducted to determine if subchronic oral exposure to trichloroethylene (TCE) influenced female reproductive performance, and if TCE or major metabolites trichloroacetic acid (TCA) and trichloroethanol (TCOH) preferentially accumulated in female reproductive organs or neonatal tissues. Female Long-Evans hooded rats were exposed to vehicle (corn-oil), 10, 100 or 1000 mg/kg/day by gavage for 2 weeks before mating and throughout mating to day 21 of pregnancy. Gas chromatography analysis of tissues from females at the end of premating exposure indicated that TCE levels were uniformly high in fat, adrenals and ovaries across treatment groups, while uterine tissue had relatively high levels of TCA. Female fertility, however, was not influenced in any treatment group. In the 1000 mg/kg/day group, 5 out of 23 females died and weight gain was significantly depressed throughout the treatment period. Neonatal survival was significantly depressed in this group alone, with the majority of deaths occurring among female offspring at the time of birth. TCA levels in blood, liver, and milk contents of the stomach in female but not male neonates increased across treatment groups. These results indicate that oral exposure to TCE at levels below those causing limiting maternal toxicity had no influence on pregnancy outcome, and that the accumulation of TCE and TCA in ovaries, adrenals and uteri had no influence on mating success.
本研究旨在确定亚慢性经口接触三氯乙烯（TCE）是否会影响雌性生殖性能，以及TCE或主要代谢产物三氯乙酸（TCA）和三氯乙醇（TCOH）是否会优先在雌性生殖器官或新生组织中蓄积。在交配前2周及整个交配期直至妊娠第21天，对雌性Long-Evans带帽大鼠经口灌胃给予赋形剂（玉米油）、10、100或1000 mg/kg/天。对交配前暴露结束时雌性大鼠组织进行气相色谱分析表明，各处理组的脂肪、肾上腺和卵巢中TCE水平均较高，而子宫组织中TCA水平相对较高。然而，任何处理组的雌性生育力均未受到影响。在1000 mg/kg/天组中，23只雌性大鼠中有5只死亡，且在整个治疗期间体重增加显著降低。仅该组的新生仔鼠存活率显著降低，大多数死亡发生在雌性仔鼠出生时。雌性而非雄性新生仔鼠的血液、肝脏和胃内容物中的TCA水平随处理组的增加而升高。这些结果表明，经口接触低于导致母体毒性限制水平的TCE对妊娠结局无影响，且TCE和TCA在卵巢、肾上腺和子宫中的蓄积对交配成功率无影响。
