Wild G, Bennett G
Am J Anat. 1984 Aug;170(4):531-43. doi: 10.1002/aja.1001700403.
Young (40 gm) rats were given a single intravenous injection of colchicine (4.0 mg) or vinblastine (2.0 mg). At 10 min after colchicine and 30 min after vinblastine administration, the rats were injected with 3H-fucose. Control rats received 3H-fucose only. All rats were sacrificed 90 min after 3H-fucose injection and their tissues processed for radioautography. In thyroid follicular cells of control animals, at this time interval, 57% of the total label was associated with colloid and secretory vesicles in the apical cytoplasm while 27% was localized in the Golgi apparatus and neighboring vesicles. In experimental animals, the proportion of label in colloid and apical vesicles was reduced by more than 69% after colchicine and more than 83% after vinblastine treatment. The proportion of label in the Golgi region, on the other hand, increased by more than 125% after colchicine and more than 179% after vinblastine treatment. Within the Golgi region, the great majority of the label was associated with secretory vesicles which accumulated adjacent to the trans face of the Golgi stacks. It is concluded that the drugs do not interfere with passage of newly synthesized thyroglobulin from the Golgi saccules to nearby secretory vesicles, but do inhibit intracellular migration of these vesicles to the cell apex. In most cells the number of vesicles in the apical cytoplasm diminished, but this was not always the case, suggesting that exocytosis may also be partially inhibited. The loss of microtubules in drug-treated cells suggests that the microtubules may be necessary for intracellular transport of thyroglobulin.
给年幼（40克）大鼠单次静脉注射秋水仙碱（4.0毫克）或长春花碱（2.0毫克）。在注射秋水仙碱10分钟后以及注射长春花碱30分钟后，给大鼠注射3H-岩藻糖。对照大鼠仅接受3H-岩藻糖。在注射3H-岩藻糖90分钟后处死所有大鼠，并对其组织进行放射自显影处理。在此时间间隔，对照动物的甲状腺滤泡细胞中，总标记物的57%与顶端细胞质中的胶体和分泌小泡相关，而27%定位于高尔基体及其相邻小泡。在实验动物中，秋水仙碱处理后胶体和顶端小泡中的标记物比例降低超过69%，长春花碱处理后降低超过83%。另一方面，秋水仙碱处理后高尔基体区域的标记物比例增加超过125%，长春花碱处理后增加超过179%。在高尔基体区域内，绝大多数标记物与聚集在高尔基体堆叠反面附近的分泌小泡相关。得出的结论是，这些药物不干扰新合成的甲状腺球蛋白从高尔基体囊泡向附近分泌小泡的转运，但确实抑制这些小泡向细胞顶端的细胞内迁移。在大多数细胞中，顶端细胞质中的小泡数量减少，但情况并非总是如此，这表明胞吐作用也可能部分受到抑制。药物处理细胞中微管的丧失表明微管可能是甲状腺球蛋白细胞内运输所必需的。
