Braun D P, Mokyr M B, Dray S
Cancer Res. 1978 Jun;38(6):1626-32.
Cultured spleen cells from normal or MOPC-315 tumor-bearing BALB/c mice that were pretreated in vivo with Bacillus Calmette-Guérin (BCG) exhibited in vitro cytotoxicity against MOPC-315 plasmacytoma. In vitro education of BALB/c spleen cells from normal or tumor-bearing mice by cocultivation with mitomycin C-treated MOPC-315 stimulator cells also resulted in antitumor cytotoxicity. The combination of BCG pretreatment of donor mice with the in vitro education of their spleen cells resulted in a level of anti-MOPC-315 cytotoxicity that was greater than the sum of the levels of cytotoxicity exhibited by spleen cells subjected to either process alone. The levels of cytotoxicity exhibited by educated or uneducated spleen cells from BCG-pretreated mice were dependent on the dose of BCG used and on the time interval between in vivo pretreatment and the initiation of in vitro culture. Thus, our findings suggest that educated spleen cells from tumor-bearing hosts that were pretreated with BCG might be useful in immunotherapeutic regimens requiring histocompatible cells with augmented antitumor cytotoxicity.
用卡介苗（BCG）进行体内预处理的正常或携带MOPC - 315肿瘤的BALB/c小鼠的培养脾细胞，在体外对MOPC - 315浆细胞瘤表现出细胞毒性。通过与丝裂霉素C处理的MOPC - 315刺激细胞共培养，对来自正常或荷瘤小鼠的BALB/c脾细胞进行体外诱导，也产生了抗肿瘤细胞毒性。供体小鼠的BCG预处理与其脾细胞的体外诱导相结合，产生的抗MOPC - 315细胞毒性水平高于单独进行任一处理的脾细胞所表现出的细胞毒性水平之和。来自BCG预处理小鼠的诱导或未诱导脾细胞所表现出的细胞毒性水平，取决于所用BCG的剂量以及体内预处理与体外培养开始之间的时间间隔。因此，我们的研究结果表明，来自经BCG预处理的荷瘤宿主的诱导脾细胞，可能在需要具有增强抗肿瘤细胞毒性的组织相容性细胞的免疫治疗方案中有用。
