Braun D P, De Boer K P, Harris J E
Department of Medicine, Rush Medical College, Chicago, Illinois 60612.
Cancer Immunol Immunother. 1982;14(2):86-91. doi: 10.1007/BF00200173.
Chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity were measured in peripheral blood mononuclear cells (PBMC) from normal subjects and solid tumor cancer patients. These functions were found to be differentially affected by malignant disease. In cancer patients with disseminated disease, indomethacin-sensitive suppression and chemiluminescence emission were increased to a level significantly higher than normal without a concurrent increase in adherent cell cytotoxic function. In cancer patients with at most minimum residual diseases, the levels of chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity found were comparable to those of the normal study population. In vitro stimulation of cells from patients with disseminated disease by phorbol myristic acetate (PMA) increased chemiluminescence overcame the suppressive effects of indomethacin-sensitive suppressor cells, and increased adherent cell cytotoxicity; in cells from patients with at most minimum residual disease, PMA increased chemiluminescence and cytotoxicity without influencing the activity of indomethacin-sensitive suppressor cells. Vaccination of lung cancer patients with Freund's complete adjuvant or Freund's complete adjuvant plus tumor antigen extracts led to increased levels of chemiluminescence and increased levels of adherent cell cytotoxicity without altering indomethacin-sensitive regulatory cell function.
在正常受试者和实体肿瘤癌症患者的外周血单核细胞（PBMC）中测量了化学发光、吲哚美辛敏感抑制和贴壁细胞细胞毒性。发现这些功能受到恶性疾病的不同影响。在患有播散性疾病的癌症患者中，吲哚美辛敏感抑制和化学发光发射增加到显著高于正常水平，而贴壁细胞细胞毒性功能没有同时增加。在至多有最小残留疾病的癌症患者中，所发现的化学发光、吲哚美辛敏感抑制和贴壁细胞细胞毒性水平与正常研究人群相当。用佛波酯（PMA）体外刺激播散性疾病患者的细胞可增加化学发光，克服吲哚美辛敏感抑制细胞的抑制作用，并增加贴壁细胞细胞毒性；在至多有最小残留疾病的患者的细胞中，PMA增加化学发光和细胞毒性，而不影响吲哚美辛敏感抑制细胞的活性。用弗氏完全佐剂或弗氏完全佐剂加肿瘤抗原提取物对肺癌患者进行疫苗接种，可导致化学发光水平升高和贴壁细胞细胞毒性水平升高，而不改变吲哚美辛敏感调节细胞功能。
