Scherini E, Mares V, Sýkorová J, Bernocchi G
Basic Appl Histochem. 1984;28(2):195-204.
DNA synthesis and Feulgen - DNA content in Purkinje neurons were examined in early postnatal rats exposed to cis-dichlorodiammineplatinum II (cis-DDP) and (3H) thymidine. Autoradiographic data revealed a significant although low labelling in control rats on postnatal day 10 to 11; the incorporation deeply decreased after cis-DDP administration. Cytophotometry showed formation of some Purkinje cells with hyperdiploid DNA content in control animals between postnatal day 11 and 16, whereas in cis-DDP treated rats all cells remained diploid. A close correlation between the formation of hyperdiploid Feulgen-DNA nuclei and the incorporation of (3H) thymidine provides new, indirect support for an actual DNA surplus in some Purkinje cells, although the incorporation of some of (3H) thymidine due to an enhanced turnover and or repair of DNA in Purkinje cells of 10 to 11 days old rats is not excluded.
在出生后早期暴露于顺式二氯二氨铂（cis-DDP）和（3H）胸腺嘧啶核苷的大鼠中，检测了浦肯野神经元中的DNA合成和福尔根DNA含量。放射自显影数据显示，在出生后第10至11天，对照大鼠有明显但较低的标记；给予顺式二氯二氨铂后，掺入量大幅下降。细胞光度测定显示，在出生后第11至16天之间，对照动物中有一些浦肯野细胞形成了超二倍体DNA含量，而在顺式二氯二氨铂处理的大鼠中，所有细胞均保持二倍体。超二倍体福尔根-DNA核的形成与（3H）胸腺嘧啶核苷的掺入之间的密切相关性为一些浦肯野细胞中实际的DNA过剩提供了新的间接支持，尽管不排除由于10至11日龄大鼠浦肯野细胞中DNA周转和/或修复增强导致的一些（3H）胸腺嘧啶核苷的掺入。
