Gottwik M G, Puschmann S, Wüsten B, Nienaber C, Müller K D, Hofmann M, Schaper W
Basic Res Cardiol. 1984 May-Jun;79(3):337-43. doi: 10.1007/BF01908034.
Previous work of this laboratory has shown that collateral flow can be increased over six weeks by a subcritical external constriction of the circumflex artery causing a 50 +/- 10% reduction of postocclusive reactive hyperemia. To investigate collateral function in acute myocardial infarction, the model was used to ligate two distant coronary branches on the ventricle simultaneously in order to compare in 8 dogs infarct size and perfusion area of the ligated vessels in control and collateralized sections. The acute collateral flow measured 7.2 +/- 2.5 ml/100 g/min-1 and increased to 17.3 +/- 6.7 (p less than 0.001) over 6 weeks. Separate analysis revealed a predominant increase of collateral flow in the epicardial layers 23.1 +/- 7.5 (p less than 0.01) versus 6.9 +/- 2.8 (p less than 0.01) in the subendocardium. Infarct size in the control area was 52.0 +/- 14.7% of the perfusion area, in the collateralized zone 19.0 +/- 14.2% (p less than 0.001). Infarct size expressed as per cent of perfusion area and collateral flow in the area at risk expressed as per cent of flow of normal sections correlated: (r = 0.76; p less than 0.05). Therefore, infarct size after a 6 hour coronary occlusion can be considered a function of the collateral flow over normal perfusion ratio. Localized induction of collaterals in this model caused a significant reduction of infarct size in relation to the perfusion area at risk.
该实验室之前的研究表明，通过对旋支动脉进行亚临界外部缩窄，使闭塞后反应性充血减少50±10%，六周内可使侧支血流增加。为研究急性心肌梗死中的侧支功能，该模型用于同时结扎心室上两条较远的冠状动脉分支，以便在8只犬中比较对照区域和侧支循环区域中结扎血管的梗死面积和灌注面积。急性侧支血流为7.2±2.5 ml/100 g/min-1，并在6周内增加至17.3±6.7（p<0.001）。单独分析显示，心外膜层侧支血流显著增加，为23.1±7.5（p<0.01），而心内膜下层为6.9±2.8（p<0.01）。对照区域的梗死面积为灌注面积的52.0±14.7%，侧支循环区域为19.0±14.2%（p<0.001）。以灌注面积百分比表示的梗死面积与以正常区域血流百分比表示的危险区域侧支血流相关：（r = 0.76；p<0.05）。因此，冠状动脉闭塞6小时后的梗死面积可被视为侧支血流与正常灌注比值的函数。该模型中侧支的局部诱导导致梗死面积相对于危险灌注区域显著减少。
