利用扩展X射线吸收精细结构获得的多巴胺β-单加氧酶铜位点的直接结构信息。
Direct structural information for the copper site of dopamine beta-mono-oxygenase obtained by using extended X-ray-absorption fine structure.
作者信息
Hasnain S S, Diakun G P, Knowles P F, Binsted N, Garner C D, Blackburn N J
出版信息
Biochem J. 1984 Jul 15;221(2):545-8. doi: 10.1042/bj2210545.
Abstract
Copper K-edge e.x.a.f.s (extended X-ray-absorption fine structure) was measured for dopamine beta-mono-oxygenase in aqueous solution. Comparison with the Cu K-edge e.x.a.f.s. of bovine erythrocyte superoxide dismutase shows a close resemblance. Detailed analysis of the e.x.a.f.s. indicates that the copper atom is bound to four imidazole groups at 0.201 nm with one or two oxygen atoms at 0.23 nm.
摘要
在水溶液中对多巴胺β-单加氧酶进行了铜K边扩展X射线吸收精细结构（e.x.a.f.s）测量。与牛红细胞超氧化物歧化酶的铜K边e.x.a.f.s进行比较，结果显示二者极为相似。对e.x.a.f.s的详细分析表明，铜原子与四个咪唑基团以0.201纳米的距离结合，同时与一个或两个氧原子以0.23纳米的距离结合。
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