Etienne J, Grüber A, Polonovski J
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1117-24. doi: 10.1016/0006-291x(84)91207-5.
Normal human serum and plasma were shown to contain a factor inhibiting phospholipase A2. This factor has been separated from human serum and plasma by chromatography on a Blue-Ultrogel column and was eluted by tris-HCl buffer (pH 7.2); the proteins eluted by 1 M NaCl-tris HCl buffer exhibited phospholipase A2 activity. This activity was abolished when the inhibitory factor was added to proteins possessing such activity. The inhibitory factor was not dialysable, sensitive to both heat and trypsin treatment, suggesting that it is a protein. In vitro, the same factor inhibited phospholipase A2 rat serum.
正常人体血清和血浆被证明含有一种抑制磷脂酶A2的因子。该因子已通过在蓝色琼脂糖凝胶柱上的色谱法从人血清和血浆中分离出来，并用三羟甲基氨基甲烷盐酸盐缓冲液（pH 7.2）洗脱；用1M氯化钠-三羟甲基氨基甲烷盐酸盐缓冲液洗脱的蛋白质表现出磷脂酶A2活性。当将抑制因子添加到具有这种活性的蛋白质中时，这种活性就会消失。该抑制因子不可透析，对热和胰蛋白酶处理均敏感，表明它是一种蛋白质。在体外，相同的因子抑制大鼠血清中的磷脂酶A2。
