Matsumura H, Daikoku S
Cell Tissue Res. 1978 Apr 28;188(3):491-6. doi: 10.1007/BF00219786.
The effects of sex-steroids on the LH-cell development in neonatal rats were studied. The cells were stained immunohistochemically by applying anti-HCG serum. On the second day after birth some of the animals of both sexes were gonadectomized and simultaneously injected with testosterone or estradiol (50 or 200 microgram). The remaining animals were either gonadectomized or injected with either one of the sex-steroids. The LH-cell numbers in each group were determined on the 12th day of age from serially cut histological sections of the pituitary. In castrated males the number of LH-cells was about twice that of the intact animals. In the so-called sex-zone, LH-cells tended to be hypertrophied in castrates. These alterations in the appearance of LH-cells did not occur after ovariectomy. In gonadectomized animals injected with sex-steroids (200 microgram), the cells were markedly reduced in number and size, both in males and females. Testosterone injection (50 microgram) into intact newborn animals also suppressed the numerical development of LH-cells, especially in females. These alterations were particularly evident in the sex-zone in both sexes. Thus the present findings show that sex-steroids may be involved in sexual differences in morphological development of LH-cells in newborn rats.
研究了性类固醇对新生大鼠促黄体生成素（LH）细胞发育的影响。通过应用抗人绒毛膜促性腺激素（HCG）血清对细胞进行免疫组织化学染色。出生后第二天，对部分雌雄动物进行性腺切除，并同时注射睾酮或雌二醇（50或200微克）。其余动物要么进行性腺切除，要么注射其中一种性类固醇。在出生后第12天，从垂体的连续组织切片中确定每组的LH细胞数量。去势雄性大鼠的LH细胞数量约为完整动物的两倍。在所谓的性区，去势大鼠的LH细胞往往肥大。卵巢切除术后未出现LH细胞外观的这些改变。在注射了性类固醇（200微克）的性腺切除动物中，无论雄性还是雌性，细胞数量和大小均明显减少。向完整的新生动物注射睾酮（50微克）也会抑制LH细胞的数量发育，尤其是在雌性动物中。这些改变在两性的性区尤为明显。因此，目前的研究结果表明，性类固醇可能参与新生大鼠LH细胞形态发育的性别差异。
