Cerulenin effect on phospholipid metabolism in Mycobacterium smegmatis ATCC 607.

Phospholipid metabolism in the presence of a subinhibitory concentration of cerulenin was studied in Mycobacterium smegmatis ATCC 607 by pulse labelling and subsequent chasing of radioactivity in phospholipids using [32P]orthophosphate. Cerulenin inhibited biosynthesis of total phospholipids to a significant level which is reflected equally in all the phospholipid components (phosphatidylethanolamine, phosphatidylinositol mannosides and cardiolipin) within the time of exposure. On chase, alteration in degradation of all phospholipid components was observed on cerulenin treatment, in comparison with control cells. Differences seen in the metabolism of phospholipids in cerulenin-treated and control cells are discussed.