Hine D G, Tanaka K
Biomed Mass Spectrom. 1984 Jul;11(7):332-9. doi: 10.1002/bms.1200110704.
Numerous abnormal metabolites were identified in large amounts in the urine of hypoglycin-treated rats using capillary gas chromatography/mass spectrometry-computer analysis. These metabolites are not detectable in significant amounts in normal rats' urine. Ten of them have not been previously associated with hypoglycin administration: these are several hydroxy compounds, including those from the valine and isoleucine pathways, 2-oxo-adipic acid, n-butyrylglycine and isovaleryl glucuronide. These results indicate that the pathways of isoleucine and valine metabolism are inhibited at their respective acyl-CoA dehydrogenation steps, as is the case for fatty acid, leucine and lysine metabolism, as previously shown. The mass spectra of the trimethylsilyl derivatives of cis, cis-4,7-decadiene-1,10-dioic, cis-4-decene-1,10-dioic, cis-4-octene-1,8-dioic acids, and (methylenecyclopropyl)acetylglycine, which were previously identified using nuclear magnetic resonance and oxidative cleavage or acid hydrolysis, are presented for the first time.
使用毛细管气相色谱/质谱联用计算机分析，在低血糖素处理的大鼠尿液中大量鉴定出多种异常代谢产物。在正常大鼠尿液中无法检测到大量的这些代谢产物。其中有10种以前未与低血糖素给药相关联：它们是几种羟基化合物，包括来自缬氨酸和异亮氨酸途径的化合物、2-氧代己二酸、正丁酰甘氨酸和异戊酰葡萄糖醛酸。这些结果表明，异亮氨酸和缬氨酸代谢途径在其各自的酰基辅酶A脱氢步骤受到抑制，脂肪酸、亮氨酸和赖氨酸代谢也是如此，如先前所示。首次展示了顺式、顺式-4,7-癸二烯-1,10-二酸、顺式-4-癸烯-1,10-二酸、顺式-4-辛烯-1,8-二酸以及（亚甲基环丙基）乙酰甘氨酸的三甲基硅烷基衍生物的质谱，这些化合物先前是通过核磁共振以及氧化裂解或酸水解鉴定出来的。
