Ramsden D B, Farmer M J
Biomed Mass Spectrom. 1984 Aug;11(8):421-7. doi: 10.1002/bms.1200110811.
The components of a gas chromatographic mass spectrometric-selected ion monitoring (SIM) assay for thyroxine (T4) in human serum are described. The internal standard for the assay was synthesized from deuterium-labelled 3,5-diiodotyrosine and 3,5-diiodo-4-hydroxyphenylpyruvic acid. A novel method was developed for isolating the products of the coupling reaction. The results obtained by gas chromatography mass spectrometry SIM were compared with those of radioimmunoassay. The gas chromatographic mass spectrometric SIM assay would form the basis of a reference assay for T4.
描述了一种用于测定人血清中甲状腺素（T4）的气相色谱 - 质谱选择离子监测（SIM）分析方法的组成部分。该分析方法的内标是由氘标记的3,5 - 二碘酪氨酸和3,5 - 二碘 - 4 - 羟基苯丙酮酸合成的。开发了一种分离偶联反应产物的新方法。将气相色谱 - 质谱SIM法获得的结果与放射免疫分析法的结果进行了比较。气相色谱 - 质谱SIM分析将构成T4参考分析方法的基础。
