兔主动脉的内皮依赖性舒张：抗氧化剂和羟基化二十碳四烯酸的作用。
The endothelium-dependent relaxation of rabbit aorta: effects of antioxidants and hydroxylated eicosatetraenoic acids.
作者信息
Förstermann U, Neufang B
出版信息
Br J Pharmacol. 1984 Aug;82(4):765-7. doi: 10.1111/j.1476-5381.1984.tb16472.x.
Abstract
Acetylcholine (ACh) induced concentration-dependent relaxations of rabbit aortic strips precontracted with noradrenaline. The relaxations were abolished if the endothelium of the strips was disrupted. Three different antioxidants (butylated hydroxytoluene, dithiothreitol and alpha-tocopherol) reversed the endothelium-dependent vasodilation in a concentration-dependent manner. However, the antioxidant ascorbic acid did not alter the vasodilatation. The hydroxylated eicosatetraenoic acids 5-HETE, 12-HETE, 15-HETE and 5,12-diHETE had no effect on aortic strips under basal or induced tension. These results suggest, that (non-cyclo-oxygenase) oxidation processes, insensitive to the action of ascorbic acid, represent a crucial step in the endothelium-dependent dilatation of rabbit aorta by ACh. The hydroxylated fatty acids tested are unlikely to mediate this relaxation.
摘要
乙酰胆碱（ACh）可使预先用去甲肾上腺素预收缩的兔主动脉条产生浓度依赖性舒张。如果主动脉条的内皮被破坏，舒张作用就会消失。三种不同的抗氧化剂（丁基化羟基甲苯、二硫苏糖醇和α-生育酚）以浓度依赖性方式逆转内皮依赖性血管舒张。然而，抗氧化剂抗坏血酸并未改变血管舒张作用。羟基化二十碳四烯酸5-HETE、12-HETE、15-HETE和5,12-二HETE在基础张力或诱导张力下对主动脉条均无作用。这些结果表明，对抗坏血酸作用不敏感的（非环氧化酶）氧化过程是ACh引起兔主动脉内皮依赖性舒张的关键步骤。所测试的羟基化脂肪酸不太可能介导这种舒张作用。
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