将公牛精液分离为携带X和Y染色体的部分对所得胚胎性别比例的影响。
Effect of separating bull semen into X and Y chromosome-bearing fractions on the sex ratio of resulting embryos.
作者信息
Hagele W C, Hare W C, Singh E L, Grylls J L, Abt D A
出版信息
Can J Comp Med. 1984 Jul;48(3):294-8.
Seventy-six, day 12 to day 15 bovine embryos, collected from 14 donors which had been inseminated with either X or Y chromosome-bearing spermatozoa fractions of semen separated by a thermal convection counterstreaming sedimentation and forced convection galvanization process, were processed for sexing by chromosomal analysis. Fifty-seven embryos were sexed; 20 from Y chromosome-bearing and 37 from X chromosome-bearing fractions of semen. Statistical analysis of the sexing data indicated that there was no significant difference in the male: female ratio for donors receiving male fractions compared to those receiving female fractions. The Y chromosome-bearing fractions produced a male: female ratio that was indistinguishable from the expected 1:1 ratio. However, the X chromosome-bearing fractions of semen produced a highly significant deviation from the expected 1:1 ratio towards the male.
从14头供体牛收集了76个第12至15天的胚胎，这些供体牛用通过热对流逆流沉降和强制对流镀锌工艺分离的含有X或Y染色体的精子部分进行授精，然后对这些胚胎进行染色体分析以确定性别。57个胚胎确定了性别；其中20个来自含有Y染色体的精子部分，37个来自含有X染色体的精子部分。对性别鉴定数据的统计分析表明，接受含雄性精子部分的供体与接受含雌性精子部分的供体相比，其雄性与雌性比例没有显著差异。含有Y染色体的精子部分产生的雄性与雌性比例与预期的1:1比例没有区别。然而，含有X染色体的精子部分产生的比例与预期的1:1比例相比，向雄性方向有高度显著的偏差。
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