Thie M, Bochskanl R, Kirchner C
Cell Tissue Res. 1984;237(1):155-60. doi: 10.1007/BF00229211.
Two antigens of the beta-glycoprotein fraction from rabbit uterine secretion of the seventh day post coitum were purified firstly by gel filtration on Sephadex G 150, then either by ion exchange chromatography on DEAE Sephacel or chromatofocusing on PBE 94. By the use of a specific antiserum, raised in female sheep, two antigens with alpha 2- and beta 2-mobility in agar gel electrophoresis could be demonstrated. Immunohistochemical staining of the uterine epithelium at the seventh and eighth day post coitum showed the antigens to be localized in a ciliated cell type of conspicuous shape, which is supposed to be the site of synthesis. Stain accumulated mainly in the apical part of the cell, but there were also small deposits around the nucleus.
对交配后第7天兔子宫分泌物中β-糖蛋白组分的两种抗原进行了纯化，首先通过在Sephadex G 150上进行凝胶过滤，然后通过在DEAE Sephacel上进行离子交换色谱或在PBE 94上进行色谱聚焦。使用在雌性绵羊中产生的特异性抗血清，可在琼脂凝胶电泳中证明两种具有α2和β2迁移率的抗原。对交配后第7天和第8天子宫上皮的免疫组织化学染色显示，抗原定位于形状明显的纤毛细胞类型中，推测该细胞类型为合成部位。染色主要积聚在细胞的顶端部分，但细胞核周围也有少量沉积物。
