从人和犬类组织中纯化肌酸激酶 - BB 同工酶。

Purification of creatine kinase-BB isoenzymes from human and canine tissues.

作者信息

Miller J, Wei R

出版信息

Clin Chim Acta. 1984 Sep 15;142(1):61-71. doi: 10.1016/0009-8981(84)90101-3.

Abstract

We describe a procedure for the purification of creatine kinase BB isoenzyme from canine and human brain tissue. The purification involves sequentially 50-70% (NH4)2SO4 fractionation, DEAE-Sephacel, Blue-Sepharose CL-6B, and chromatofocusing chromatography. This method produces a homogeneous protein purification as verified by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing chromatography.

摘要

我们描述了一种从犬类和人类脑组织中纯化肌酸激酶BB同工酶的方法。纯化过程依次包括50%-70%硫酸铵分级分离、DEAE-琼脂糖凝胶、蓝色琼脂糖凝胶CL-6B和层析聚焦色谱法。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和等电聚焦色谱法验证，该方法可产生均一的蛋白质纯化产物。