Thomas J M, Crisp M, Hodes M E
Clin Chim Acta. 1984 Sep 15;142(1):73-81. doi: 10.1016/0009-8981(84)90102-5.
Ribonuclease (RNase) activity from human serum appears as multiple zones of activity following isoelectric focusing in thin layer polyacrylamide gel. At least one but not all of these zones is cross reactive with rabbit antibovine pancreatic RNase A antiserum. Treatment of serum or partially purified serum RNase with neuraminidase reduces the complexity of the serum RNase banding pattern to a major band which focuses at a pH of 9.5 or greater and a minor zone of activity which focuses at about pH 6.0-6.2. Trypsin does not affect the pattern. Thus, sialic acid residues account for a large portion of the heterogeneity of human serum RNase. Neuraminidase treatment is requisite for evaluating RNase from serum and certain other sources.
人血清中的核糖核酸酶（RNase）活性在薄层聚丙烯酰胺凝胶中进行等电聚焦后呈现为多个活性区带。这些区带中至少有一个但并非全部与兔抗牛胰腺RNase A抗血清发生交叉反应。用神经氨酸酶处理血清或部分纯化的血清RNase，可将血清RNase条带模式的复杂性降低为一条主要条带，其聚焦于pH 9.5或更高，以及一个次要活性区带，其聚焦于约pH 6.0 - 6.2。胰蛋白酶不影响该模式。因此，唾液酸残基占人血清RNase异质性的很大一部分。神经氨酸酶处理对于评估血清和某些其他来源的RNase是必需的。
