Grinstein S, Furuya W
Comp Biochem Physiol B. 1984;78(3):657-62. doi: 10.1016/0305-0491(84)90114-7.
The major glycoproteins of porcine platelet plasma membranes and alpha-granule membranes were compared. Significant cross-contamination of the two preparations was ruled out by surface labeling and proteolysis experiments. At least four of the major glycoproteins of the alpha-granule membrane had counterparts in the plasma membranes with identical molecular weight and lectin-binding properties. Two of these (mol. wts 110,000 and 125,000) were further analysed by one-dimensional peptide mapping. The results confirmed that there are two distinct pools of identical glycoproteins: one on the surface membrane and the other on the alpha-granular membrane. The 110,000 and 125,000 mol. wt glycoproteins are probably equivalent to glycoproteins IIb and IIIa of the human platelet and may therefore be involved in fibrinogen binding.
对猪血小板质膜和α-颗粒膜的主要糖蛋白进行了比较。通过表面标记和蛋白水解实验排除了两种制剂之间明显的交叉污染。α-颗粒膜的至少四种主要糖蛋白在质膜中有分子量和凝集素结合特性相同的对应物。其中两种（分子量分别为110,000和125,000）通过一维肽图谱进一步分析。结果证实存在两个不同的相同糖蛋白池：一个在表面膜上，另一个在α-颗粒膜上。分子量为110,000和125,000的糖蛋白可能相当于人血小板的糖蛋白IIb和IIIa，因此可能参与纤维蛋白原结合。
