Wenk G L, Cribbs B, McCall L
Exp Brain Res. 1984;56(2):335-40. doi: 10.1007/BF00236289.
The present experiment was designed to determine the optimal placement of ibotenic acid in the nucleus basalis magnocellularis (NBM) in order to get a substantial destruction of cholinergic input to the frontolateral cortex, while sparing that to the hippocampus. Two different volumes of ibotenic acid were injected at four different placements in the basal forebrain. Choline acetyltransferase (ChAT) levels were measured in three regions of neocortex and two regions of hippocampus. Lesions in the NBM produced significant decreases in neocortical ChAT (up to 54%), with the greatest and most extensive effects being produced by lesions that were more lateral and caudal (0.9 mm posterior to Bremga; 2.6 mm lateral to the midline). All lesions that were placed 0.4 mm posterior to bregma, or placed 2.0 mm lateral to the midline, produced significant decreases in ChAT activity levels in the ventral hippocampus. The results show that the specific coordinates and volumes of ibotenic acid are critical to produce selective and substantial destruction of cells in the NBM. These findings have substantial implications for the interpretation of results of experiments examining the behavioral changes that occur following injections of ibotenic acid into the NBM.
本实验旨在确定鹅膏蕈氨酸在基底大细胞核（NBM）中的最佳注射位置，以便在保留对海马胆碱能输入的同时，对额叶外侧皮质的胆碱能输入进行实质性破坏。在基底前脑的四个不同位置注射了两种不同体积的鹅膏蕈氨酸。在新皮质的三个区域和海马的两个区域测量了胆碱乙酰转移酶（ChAT）水平。NBM中的损伤导致新皮质ChAT显著降低（高达54%），最外侧和最靠后的损伤（距前囟后0.9 mm；距中线外侧2.6 mm）产生的影响最大且最广泛。所有位于前囟后0.4 mm或距中线外侧2.0 mm的损伤均导致腹侧海马ChAT活性水平显著降低。结果表明，鹅膏蕈氨酸的特定坐标和体积对于在NBM中产生选择性和实质性的细胞破坏至关重要。这些发现对于解释将鹅膏蕈氨酸注射到NBM后发生的行为变化的实验结果具有重要意义。
