Solursh M, Jensen K L, Zanetti N C, Linsenmayer T F, Reiter R S
Dev Biol. 1984 Oct;105(2):451-7. doi: 10.1016/0012-1606(84)90302-6.
It has been previously observed that single chick embryonic limb mesenchymal cells can differentiate into chondrocytes without cell-cell interactions when cultured in collagen or agarose gels. In the present study, limb ectoderm, but not dermis, inhibits chondrogenesis when placed on such collagen gel cultures. The inhibitory influence can be transmitted extensive distances in the gel, even when the ectoderm is placed on a porous filter. Collagen gels, preconditioned with limb ectoderms, are also inhibitory to chondrogenesis. On the other hand, chondrogenesis is less inhibited by ectoderm when the mesenchymal cells are placed in agarose. These results suggest that the antichondrogenic effect of limb ectoderm is mediated through alterations of the collagenous extracellular matrix and support the idea that the extracellular matrix must be considered as an organized, functional unit capable of regulating cell differentiation.
先前已经观察到，当在胶原蛋白或琼脂糖凝胶中培养时，单个鸡胚肢体间充质细胞无需细胞间相互作用就能分化为软骨细胞。在本研究中，肢体外胚层而非真皮层，放置在这种胶原蛋白凝胶培养物上时会抑制软骨形成。即使外胚层放置在多孔滤器上，这种抑制作用也能在凝胶中远距离传递。预先用肢体外胚层处理过的胶原蛋白凝胶也会抑制软骨形成。另一方面，当间充质细胞置于琼脂糖中时，外胚层对软骨形成的抑制作用较小。这些结果表明，肢体外胚层的抗软骨形成作用是通过胶原细胞外基质的改变介导的，并支持这样一种观点，即细胞外基质必须被视为一个能够调节细胞分化的有组织的功能单位。
