陆生等足动物膨腹土甲的高血糖神经肽。I. 分离与特性鉴定。

The hyperglycemic neuropeptide of the terrestrial isopod, Porcellio dilatatus. I. Isolation and characterization.

作者信息

Martin G, Keller R, Kegel G, Besse G, Jaros P P

出版信息

Gen Comp Endocrinol. 1984 Aug;55(2):208-16. doi: 10.1016/0016-6480(84)90103-5.

Abstract

From isolated sinus glands of Porcellio dilatatus, a hyperglycemic neuropeptide (CHH) was purified by means of a single, two-step purification procedure which consisted of gel chromatography on Sephadex G-50, followed by high-performance liquid chromatography. The 5800- to 6100-Da peptide contains 50-52 amino acids residues. The amino acid composition is (Pro, Ile)1; His1-2; (Thr, Ser, Gly, Met, Tyr)2; (Val, Phe, Lys)3; (Ala, 1/2Cys, Leu, Arg)4; Glx5; Asx7; Trp, n.d. The amino acid composition differs from that of two decapod CHHs analyzed thus far. The N-terminus is blocked. The neuropeptide exhibits little or no interspecific hyperglycemic effect in the brachyuran, Uca pugilator, and its cross-reactivity and potency in the RIA for Carcinus-CHH is very low.

摘要

从膨腹体的分离窦腺中，通过单一的两步纯化程序纯化出一种高血糖神经肽（CHH），该程序包括在葡聚糖G - 50上进行凝胶色谱，随后进行高效液相色谱。这种5800至6100道尔顿的肽含有50至52个氨基酸残基。氨基酸组成是（脯氨酸、异亮氨酸）1；组氨酸1至2；（苏氨酸、丝氨酸、甘氨酸、甲硫氨酸、酪氨酸）2；（缬氨酸、苯丙氨酸、赖氨酸）3；（丙氨酸、1/2半胱氨酸、亮氨酸、精氨酸）4；谷氨酰胺5；天冬酰胺7；色氨酸，未检出。该氨基酸组成与迄今为止分析的两种十足目CHH不同。N端被封闭。该神经肽在短尾蟹招潮蟹中几乎没有种间高血糖作用，并且其在Carcinus - CHH放射免疫分析中的交叉反应性和效力非常低。