The effects of modifying incubation on prolactin secretion in free-living pied flycatchers.

Plasma prolactin concentrations in female pied flycatchers are high during incubation, but fall from 156 +/- 27 to 67 +/- 6 ng/ml (P less than 0.01) between 2 and 4 days after the young hatch. The timing of the fall in prolactin could be determined by factors associated with the hatching of the eggs and growth of the offspring; alternatively, prolactin may remain high for a fixed period (16 days) after the onset of incubation. To distinguish between these possibilities, eggs were exchanged between nest boxes to ensure incubation periods of either 10, 14, or 19 days by different females. The results showed that the period of high prolactin secretion was shortened by 2-4 days where the eggs hatched early, but, conversely, was not lengthened in those females with extended incubation periods, the levels actually falling in these birds before the eggs hatched. There was, however, a secondary rise in prolactin 3 days after hatching, when the level of 114 +/- 11 ng/ml 22 days after the onset of incubation was much higher than that (30 +/- 4 ng/ml) at a comparable stage in the control females. Thus, high prolactin secretion cannot be maintained for longer than 16-17 days in incubating birds, but newly hatched, naked young can stimulate prolactin release in female flycatchers over a flexible period.