Gona O
Gen Comp Endocrinol. 1984 Aug;55(2):289-94. doi: 10.1016/0016-6480(84)90114-x.
Although evidence has been presented that the hormone prolactin specifically bind to the kidneys of the teleost Sarotherodon mossambicus (J. N. Fryer, 1979, Gen. Comp. Endocrinol. 39, 397-403.) prolactin has not previously been shown to become localized at any particular morphological sites in fishes. This problem has been investigated in the dwarf gourami, Colisa lalia. Ovine prolactin was labeled with (125)I and injected intramuscularly. Fish were sacrificed after 15, 30, and 90 min. Other animals were injected with an excess of unlabeled prolactin, growth hormone, or luteinizing hormone 2 min prior to administration of the label. Paraffin-embedded tissues were sectioned, coated with Kodak NTB-3, and exposed for 3-5 weeks. After developing, samples of several body tissues were stained and examined for the presence of silver grains. The results indicate that, as in rat and frog, kidney tubules of C. lalia are sites of prolactin localization. No other sites of prolactin localization were identified.
尽管已有证据表明催乳素能特异性结合于硬骨鱼莫桑比克罗非鱼的肾脏（J. N. 弗莱尔，1979年，《普通与比较内分泌学》第39卷，第397 - 403页），但此前尚未有研究表明催乳素会在鱼类的任何特定形态学部位定位。本研究以侏儒斗鱼（Colisa lalia）为对象对该问题进行了探究。用¹²⁵I标记绵羊催乳素并进行肌肉注射。分别在注射后15分钟、30分钟和90分钟处死实验鱼。其他动物在注射标记物前2分钟注射过量未标记的催乳素、生长激素或促黄体生成素。将石蜡包埋的组织切片，涂上柯达NTB - 3乳剂，并曝光3 - 5周。显影后，对多个身体组织样本进行染色，并检查是否存在银粒。结果表明，与大鼠和青蛙一样，侏儒斗鱼的肾小管是催乳素定位的部位。未发现其他催乳素定位部位。
