通过抑制法和电泳法测定碱性磷酸酶同工酶的比较。
Comparison of alkaline phosphatase isoenzymes determined by an inhibition method and by electrophoresis.
作者信息
Puukka R
出版信息
Clin Chim Acta. 1978 Apr 17;85(2):111-4. doi: 10.1016/0009-8981(78)90229-2.
A chemical inhibition procedure suitable for the routine determination of alkaline phosphatase (AP) isoenzymes in serum has been adapted for use with a fast kinetic analyzer, System Olli 3000. The results of this procedure are compared with the electrophoretic separation of alkaline phosphatase isoenzymes. The comparison of the results obtained indicates that the AP-urea/AP ratio can be used to differentiate between patients with bone and liver disease and that it is possible to estimate the relative bone and liver isoenzyme activities from this ratio quickly using two simple equations.
一种适用于常规测定血清中碱性磷酸酶（AP）同工酶的化学抑制法已被改编用于快速动力学分析仪System Olli 3000。该方法的结果与碱性磷酸酶同工酶的电泳分离结果进行了比较。所得结果的比较表明，AP-尿素/AP比值可用于区分骨病和肝病患者，并且可以使用两个简单的方程式从该比值快速估算相对的骨和肝同工酶活性。
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